Biology

We are broadly interested in the molecular mechanisms underlying skeletal muscle formation, degeneration, and regeneration. We also have a growing interest in the biology of the myotendinous junction. Current projects focus on the spatiotemporal regulation of proteases and focal adhesion proteins. We utilize a diverse set of model systems, including C2C12 cells, primary myoblasts, patient-derived iPSCs/fibroblasts, and zebrafish.

Technology

We employ existing and new chemical biology techniques in addition to standard molecular approaches. For example, we are leveraging activity-based proteomics and N-terminomics to identify and understand proteolytic switches involved in myogenic differentiation. We are also developing a genetic manipulation-free interactome mapping platform for cultured cells, zebrafish, and mammalian tissues. (See this pre-print for more.)